Recombinant:Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350-NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HB-EGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13., , , Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).
Article Title: Pharmacological Impacts of Mucopolysacccharide Polyphosphates in the Epidermis Involves Inhibition of Amphiregulin-Mediated Signals in Keratinocytes.
Article Snippet: HEKa cells (Thermo Fisher Scientific, Waltham, MA, USA) were maintained in HuMedia- KG2 (Kurabo Industries, Osaka, Japan) containing human epidermal growth factor (0.1 ng/mL), insulin (10 μg/mL), hydrocortisone (0.67 μg/mL), gentamicin (50 μg/mL), amphotericin B (50 ng/mL) and bovine brain pituitary extract (0.4% v/v) at 37°C in a humidified atmosphere containing 5% CO2. .. After HEKa cells had been seeded on cell culture plates at a density of 5.6 × 104 cells/cm2 and incubated for 24 h, then they were incubated with or without MPS (Maruho, Osaka, Japan), recombinant human amphiregulin (R&D systems, Minneapolis, MN, USA), human amphiregulin antibody (R&D systems), recombinant human EGF (R&D systems), recombinant human HBEGF (R&D systems), recombinant human EREG (R&D systems), in HuMedia- KB2 (Kurabo Industries) for 24 h at 37°C. ..
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
Article Title: Method for prediction of susceptibility to sorafenib treatment by using SULF2 gene, and composition for treatment of cancer comprising SULF2 inhibitor
Article Snippet: SULF2 antibodies were purchased from Novus Biologicals and EGFR, p-EGFR (Tyr1068), ERK (1/2), p-ERK (1/2), AKT, p-AKT, STAT1, p-STAT1 (Tyr701), STAT3, p-STAT3 (Tyr705), IκBα, LCN2, p-FGFR (Tyr653/654), FGFR and actin antibodies were purchased from Cell Signaling Biotechnology (Danvers, Mass., USA), and amphiregulin, p65 and U-0126 antibodies were purchased from Santa Cruz Biotechnology (Dallas, Tex., USA). .. Sorafenib was purchased from LC Laboratories (Woburn, Mass., USA), recombinant human amphiregulin was purchased from R & D systems (Minneapolis, Minn., USA), AEW541 was purchased from AdoQ bioscience (Burlington, Canada), gefitinib and PD173074 were purchased from Tocris Bioscience (Bristol, UK), and OKN-007 and LBW242 were purchased from MedKoo Biosciences (Chapel Hill, N.C., USA). .. Expression Constructs and Lentiviral Vector Transfection A lentiviral construct expressing STAT1 shRNA, p65 shRNA and LCN2 shRNA was purchased from Sigma-Aldrich.
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350- NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HBEGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13.25,28,41,42 Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).43 Protein expression and phosphorylation were assessed using a capillary electrophoresis method with a Wes automated system (ProteinSimple, San Jose, CA, USA), according to the manufacturer’s instructions.
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
Article Title: Mitophagy defects exacerbate inflammation and aberrant proliferation in lymphocytic thyroiditis
Article Snippet: Mitochondrial division inhibitor 1 (Mdivi-1) was purchased from Sigma-Aldrich (M0199; Saint Louis, MO, USA). .. Recombinant human amphiregulin (AREG, 262-AR-100) was purchased from R&D Systems (Minneapolis, MN, USA). .. Cetuximab (5 mg/ml) used was Erbitux (C225; SigmaAldrich, Saint Louis, MO, USA).
Incubation:Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350-NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HB-EGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13., , , Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).
Article Title: Pharmacological Impacts of Mucopolysacccharide Polyphosphates in the Epidermis Involves Inhibition of Amphiregulin-Mediated Signals in Keratinocytes.
Article Snippet: HEKa cells (Thermo Fisher Scientific, Waltham, MA, USA) were maintained in HuMedia- KG2 (Kurabo Industries, Osaka, Japan) containing human epidermal growth factor (0.1 ng/mL), insulin (10 μg/mL), hydrocortisone (0.67 μg/mL), gentamicin (50 μg/mL), amphotericin B (50 ng/mL) and bovine brain pituitary extract (0.4% v/v) at 37°C in a humidified atmosphere containing 5% CO2. .. After HEKa cells had been seeded on cell culture plates at a density of 5.6 × 104 cells/cm2 and incubated for 24 h, then they were incubated with or without MPS (Maruho, Osaka, Japan), recombinant human amphiregulin (R&D systems, Minneapolis, MN, USA), human amphiregulin antibody (R&D systems), recombinant human EGF (R&D systems), recombinant human HBEGF (R&D systems), recombinant human EREG (R&D systems), in HuMedia- KB2 (Kurabo Industries) for 24 h at 37°C. ..
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350- NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HBEGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13.25,28,41,42 Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).43 Protein expression and phosphorylation were assessed using a capillary electrophoresis method with a Wes automated system (ProteinSimple, San Jose, CA, USA), according to the manufacturer’s instructions.
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
Allele-specific Oligonucleotide:Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350-NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HB-EGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13., , , Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350- NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HBEGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13.25,28,41,42 Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).43 Protein expression and phosphorylation were assessed using a capillary electrophoresis method with a Wes automated system (ProteinSimple, San Jose, CA, USA), according to the manufacturer’s instructions.
Expressing:Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350-NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HB-EGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13., , , Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), CXCL12 (350- NS-010), delta-like canonical notch ligand 1 (1818-DL-050), HBEGF-like growth factor (259-HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA) as candidate ligands of SYT13.25,28,41,42 Each protein (0, 1, 10, 100, or 1,000 ng/mL) was added to MKN1 cells (5,000 cells per well), and the levels of SYT13mRNA were determined after incubation for 72 h. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression. .. Phosphorylation of 1,006 unique sites among 409 proteins in these cells was quantified using the PTMScan Direct Multi-Pathway Enrichment Kit (Cell Signaling Technology, Danvers, MA, USA).43 Protein expression and phosphorylation were assessed using a capillary electrophoresis method with a Wes automated system (ProteinSimple, San Jose, CA, USA), according to the manufacturer’s instructions.
Cell Culture:Article Title: Pharmacological Impacts of Mucopolysacccharide Polyphosphates in the Epidermis Involves Inhibition of Amphiregulin-Mediated Signals in Keratinocytes.
Article Snippet: HEKa cells (Thermo Fisher Scientific, Waltham, MA, USA) were maintained in HuMedia- KG2 (Kurabo Industries, Osaka, Japan) containing human epidermal growth factor (0.1 ng/mL), insulin (10 μg/mL), hydrocortisone (0.67 μg/mL), gentamicin (50 μg/mL), amphotericin B (50 ng/mL) and bovine brain pituitary extract (0.4% v/v) at 37°C in a humidified atmosphere containing 5% CO2. .. After HEKa cells had been seeded on cell culture plates at a density of 5.6 × 104 cells/cm2 and incubated for 24 h, then they were incubated with or without MPS (Maruho, Osaka, Japan), recombinant human amphiregulin (R&D systems, Minneapolis, MN, USA), human amphiregulin antibody (R&D systems), recombinant human EGF (R&D systems), recombinant human HBEGF (R&D systems), recombinant human EREG (R&D systems), in HuMedia- KB2 (Kurabo Industries) for 24 h at 37°C. ..
Binding Assay:Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
Protein-Protein interactions:Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
Article Title: Amido-Bridged Nucleic Acid-Modified Antisense Oligonucleotides Targeting SYT13 to Treat Peritoneal Metastasis of Gastric Cancer
Article Snippet: .. Recombinant human amphiregulin (262-AR-100), C-X-C motif chemokine ligand 12 (CXCL12, 350-NS-010), delta like canonical notch ligand 1 (1818-DL-050), heparin binding EGF like growth factor (HB-EGF, 259- HE-050), and jagged canonical notch ligand 1 (1277-JG-050) were obtained from R&D Systems (Minneapolis, MN, USA).13-16 Each protein (0, 1, 10, 100, or 1000 ng/ml) was added to MKN1 cells (5000 cells per well), and the levels of SYT13 mRNA were determined after incubation for 72 h. We employed two array systems to investigate intracellular signaling pathways. .. The Human XL Oncology Array Kit (R&D Systems, Minneapolis, MN, USA) was used to determine the relative levels of 84 human cancer-related proteins expressed by ASO-transfected NUGC4 cells in the presence and absence of SYT13 expression.
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